Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a kDa homodimeric protein consisting of two amino acid polypeptide subunits. Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM. AA Sequence (monomer): MKEVSEHCSH MIGNGHLKVL Gibco™ Human GM-CSF Protein, Catalog # PHC Tested in Western Blot (WB), Immunohistochemistry (IHC), ELISA (ELISA), Control (Ctrl) and Functional Assay (FN) applications. Supplied as 1 mg purified protein. Human GM-CSF Recombinant Protein. 5 References View all (16) GM-CSF proteins Datasheet Tech Support M-CSF Protein, Human (HEK, His) is the recombinant human-derived M-CSF protein, expressed by HEK, with C-His labeled tag. The total length of M-CSF Protein, Human (HEK, His) is a.a., with molecular weight of kDa. 研究背景. M-CSF 蛋白是一种重要的细胞因子，参与调节造血前体细胞（特别是巨噬细胞 Recombinant Human M-CSF. Cat# HHM-MC. Product Specifications. Expression of Human Proteins in Human Cells. Extreme low Endotoxin. High Purity. Animal Free and
Recombinant Human M-CSF_细胞生长因子_细胞生物学_索莱宝
The proliferation of murine M-NFS cells (Mouse Myeloid Leukemia indicator cell line). Reconstitution Brieflycentrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing % endotoxin-free recombinant human serum albumin Description. Specification. Reviews (0) Product Specifications. Expression of Human Proteins in Human Cells. Extreme low Endotoxin. High Purity. Animal Free and Xeno Production: Recombinant human M–CSF is produced in HEK cells and purified via sequential chromatography. Reconstitution Recommendation: Lyophilized human M-CSF induces RANKL production in mature human osteoclasts; consequently, M-CSF is a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF in M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages. Applications Reported: SAFE™ Human M-CSF Recombinant Animal Free Protein is biologically avtive. Applications Tested: The ED50 of this protein, as measured by proliferation of M-NFS cells, is Applications Reported: Recombinant human M-CSF is biologically active and can promote proliferation of M-NFS cells in culture. Applications Tested: The bioactivity of this protein, as determined in a cell proliferation assay on M-NFS cells, is 3 ng/mL. This corresponds to a specific activity of X 10e5 Plasmid and strain. The human g-csf gene was synthesized using the PCR-based gene construction method [] and inserted into plasmid pETa(+) plasmid (Novagen, USA) between the restriction sites BamHI and NdeI to construct plasmid pET-gcsf. The pET-gcsf plasmid was introduced into E. coli BL21(DE3) cells (Novagen, USA) SDS-PAGE of Human M-CSF, premium grade under reducing (R) and non-reducing (NR) conditions. Recombinant human M-CSF can regulate the proliferation,
M-CSF蛋白, Human (HEK293, His) | MCE - MCE-生物活性分子大师
Catalog # Cytokines Human Recombinant M-CSF Macrophage colony-stimulating factor 10 µg µg µg Product Description Macrophage colonystimulating factor (MCSF) is a homodimeric glycoprotein growth factor that regulates proliferation and M-CSF stands for macrophage colony-stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional [HOST] GMP Recombinant Human M-CSF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, Granulocytes and the monocytes- macrophages. M-CSF induces cells of the monocyte/ macrophage lineage. It plays a role in immunological defenses, bone metabolsm, ATP measurement. M-CSF and GM-CSF macrophages were stimulated for 15 minutes, 30 minutes, 1 hour, or 2 hours with 20 μM ATP (A; Sigma-Aldrich). The level of ATP was measured in macrophage supernatants collected at each time point using the Promega ATPlite reagent to measure ATP degradation by macrophages Macrophage Colony-Stimulating Factor human (MCSF); M-CSF, HEK cells, cell culture; Suitable for mammalian cell culture; M-CSF is encoded by the gene mapped to human chromosome 1p13; Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferati GM-CSF Protein, Human Recombinant Animal Free. View Price and Availability. The biological activity of Human M-CSF was determined in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS (Halenbeck, R. et al., , Biotechnology - ). The predicted molecular weight of Recombinant Human M-CSF is Mr kDa. However, the actual molecular weight as observed by migration on Recombinant Human M-CSF Protein Summary. Product Specifications. Purity. >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level. 靶标信息. The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with