Tech Support. Testing Data (2) Application. FIGURE: 1 / 2. Human M-CSF, Animal-Free Protein (AFUG) in FN. Bioassay analysis of Human M-CSF, Animal-Free Recombinant Protein, PeproTech® (Product # AFMG). Product Details. Applications. Tested Dilution. Publications. Functional Assay (FN) Assay The self-renewing macrophages were readily obtained by long-term culture of mouse bone marrow cells with macrophage colony-stimulating factor (M-CSF), a key cytokine for macrophage development Product name. Recombinant human M-CSF protein (Active) See all M-CSF proteins and peptides. Biological activity. Fully active compared to a standard. The ED 50 as determined by the dose-dependant proliferation of MNFS cells is ng/mL corresponding to a Specific Activity of x 10 5 IU/mg. Purity. >= 95 % SDS Product Information. Product Usage Information. Reconstitution: With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or % FBS to a final hCSF-1/M-CSF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing Recombinant human M-CSF is a kDa homodimeric protein containing two amino acid polypeptide subunits. Synonyms: Macrophage Colony Stimulating 30 publications. M-NFS cell proliferation induced by human M-CSF. Description. M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF is constitutively present at biologically active concentrations in human serum Key features and details. Expression system: Escherichia coli. Purity: > 95% SDS-PAGE. Endotoxin level Key features and details. Expression system: Escherichia coli. Purity: > 95% SDS-PAGE. Endotoxin level: Recombinant human M-CSF protein (Active)
Construction of Recombinant Human GM-CSF and GM-CSF-ApoA-I Fusi…
/bbb We expressed human macrophage colony-stimulating factor (M-CSF) in Chinese hamster ovary (CHO) cells by introducing an expression Recombinant Human M-CSF. Catalog Number: MCC. DESCRIPTION. Source. Chinese Hamster Ovary cell line, CHO derived Glu33 Arg Accession # P N terminal Sequence Glu Analysis Structure / Form. Disulfide linked homodimer. Predicted Molecular. kDa (monomer) Mass. PREPARATION AND STORAGE Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. MACS GMP Recombinant Human M-CSF is designed for ex vivo cell culture processing Overview. Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of Recombinant human macrophage colony-stimulating factor (M-CSF), a disulfide-linked dimeric protein containing 18 cysteines, has been purified in monomeric form from E. coli and renatured to
Product Information Sheet Recombinant Human M-CSF
Recombinant human M-CSF induces proliferation of M-NFS mouse myelogenous leukemia lymphoblast cells in a dose-dependent manner with an ED 50 range of - 3 ng/mL. Stability Testing for Recombinant Human M-CSF. Recombinant human M-CSF was aliquoted in PBS at mg/mL Recombinant human M-CSF induces proliferation of M-NFS mouse myelogenous leukemia lymphoblast cells in a dose-dependent manner with an Recombinant human M-CSF is a kDa homodimeric protein containing two amino acid polypeptide subunits. Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM Product Information. Product Usage Information. Reconstitution: With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or % FBS Currently, there are two recombinant GM-CSF preparations on the pharmaceutical market: Sargramostim and Molgramostim, which contain recombinant GM-CSF produced by Saccharomyces cerevisiae yeast and Escherichia coli bacteria, respectively Recombinant Human M-CSF. Catalog Number: MCC. DESCRIPTION. Source. Chinese Hamster Ovary cell line, CHO derived Glu33 Arg Accession # Tech Support. Testing Data (2) Application. FIGURE: 1 / 2. Human M-CSF, Animal-Free Protein (AFUG) in FN. Bioassay analysis of Human M-CSF,