Target Information. The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with In this study, two strains of the yeast P. pastoris were constructed, one of which produced authentic recombinant human granulocyte-macrophage colony-stimulating factor (ryGM-CSF), and the other was a chimera consisting of ryGM-CSF genetically fused with mature human apolipoprotein A-I (ApoA-I) (ryGM-CSF-ApoA-I). Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF Human Recombinant M-CSF Macrophage colony-stimulating factor 10 μg μg μg Colony stimulating factor 1, CSF-1 Product Description Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation M-CSF also enhances macrophage killing of tumor cells and microorganisms and regulates cytokine production from macrophages. Applications Reported: SAFE™ Human M-CSF Recombinant Animal Free Protein is biologically avtive. Applications Tested: The ED50 of this protein, as measured by proliferation of M-NFS cells, is We characterized rabbit macrophages that were polarized using human recombinant GM ‐ CSF and M‐ CSF. GM ‐ CSF ‐treated macrophages had higher mRNA expression of proinflammatory cytokines (M1 phenotype) than did the M‐ CSF ‐treated counterpart. By contrast, high levels of TGF ‐β and IL ‐10 expression (M2 phenotype)
Recombinant Mouse GM-CSF Protein - Novus Biologicals
The specific activity of Human M-CSF is x 10^5 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard. Purity > 95 % SDS-PAGE. Endotoxin level = Eu/µg. Expression system. Escherichia coli. Accession. P Protein length. Protein fragment. Animal free. No. Nature. Recombinant Plasmid and strain. The human g-csf gene was synthesized using the PCR-based gene construction method [] and inserted into plasmid pETa(+) plasmid (Novagen, USA) between the restriction sites BamHI and NdeI to construct plasmid pET-gcsf. The pET-gcsf plasmid was introduced into E. coli BL21(DE3) cells (Novagen, USA) Human macrophage colony-stimulating factor (M-CSF) is a homodimeric cytokine that is a member of a structurally related family of hormones defined by an unusual up-up-down-down alpha-helical bundle. To identify regions on the surface of M-CSF that might interact with the M-CSF receptor, single and d Human GM-CSF optimized for expression in P. pastoris cells and designated as ryGM-CSF was synthesized by DNA (USA) and cloned into plasmid pD at the XhoI and SalI sites. We used the plasmid pD/ryGM-CSF as the source of the ryGM-CSF gene to construct the recombinant plasmids pPICZ -A-rhGM-CSF and pPICZ -A/rhGM-CSF-link CSF-1 is required for normal male and female fertility and promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. it also plays a role in lipoprotein clearance. Recombinant Human Macrophage Colony-Stimulating Factor is produced by our Mammalian expression system and the Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a kDa homodimeric protein consisting of two amino acid polypeptide subunits. Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM. AA Sequence (monomer): MKEVSEHCSH Recombinant Mouse GM-CSF (Catalog # ML) stimulates cell proliferation of the DA3 mouse myeloma cell line. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM‑CSF is fully active on mouse cells (14, 15). Martinez-Moczygemba, M. and D.P. Huston () J 靶标信息. The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with
Recombinant Human M-CSF
It is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Recombinant human M-CSF is a kDa homodimeric and glycosylated. References. Kawasaki ES, et al (). Science (): Stein J, Borzillo GV, Rettenmier CW (). Blood 76 (7): The biological activity of Human M-CSF was determined in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS (Halenbeck, R. et al., , Biotechnology - ). The predicted molecular weight of Recombinant Human M-CSF is Mr kDa. However, the actual molecular weight as observed by migration on M-CSF stands for macrophage colony-stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional [HOST] GMP Recombinant Human M-CSF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, Macrophage Colony-Stimulating Factor human (MCSF); M-CSF, HEK cells, cell culture; Suitable for mammalian cell culture; M-CSF is encoded by the gene mapped to human chromosome 1p13; Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferati GM-CSF Protein, Human Recombinant Animal Free. View Price and The macrophage colony-stimulating factor (M-CSF) is a potent hematopoietic cytokine that is involved in diverse processes, such as regulation of inflammatory responses, bone