Association of DNA-binding domain of protein with DNA is energetically driven, where binding with major groove is an enthalpic reaction, while minor groove association is characterized by favorable entropy and unfavorable enthalpy (Privalov et al. ). Difference in energetic profile is contributed by distinct hydration pattern of both Major and Minor Grooves of the DNA. Types of DNA on the basis of forms. 1. A-form. 2. B-form. 3. Z-form. Types of DNA on the basis of location. 1. Nuclear DNA. 2. Mitochondrial DNA. Functions of DNA (Deoxyribonucleic acid) References. Properties of DNA (Deoxyribonucleic acid) Hello friends welcome back to our channel KBS biology. We will discuss about why major and minor groove formed?? details discussed in this [HOST] know this Dinucleotides. DNA molecule. Hydrogen bonds: bases pairin. Major groove and minor groove. Non-B DNA. Z-DNA. Cruciform DNA and hairpin DNA. H-DNA or triplex DNA. G4-DNA. III. Quaternary structure of the molecule - Chromatin. IV. Various. DNA and mitochondria. DNA denaturation The B-DNA major and minor groove dimensions are crucial for DNA-protein interactions. It has long been thought that the groove dimensions depend on the DNA sequence, however this relationship has remained elusive. Here, our aim is to elucidate how the DNA sequence intrinsically shapes the grooves. For both TA and CG repeats, lysine peptides bind to the minor groove of DNA more strongly than spermine molecules do (compare Figure 5 F and G), possibly because the long branched side chains of The free DNA propensity to adopt specific groove dimensions is exploited by DNA-binding proteins, as shown by the relation between TRX scores and minor groove width in protein-bound DNA. This relationship can now be seen as broadly general, but it was already incipient in isolated case studies of DNA-protein interactions, both specific
Minor Groove-Binding Architectural Proteins: Structure, Function, …
The major and minor groove width of DNA is almost constant for all the system throughout the simulation (Figs. 3 b and c), however, the average value of the minor and major groove width of DNA in GdmCl is marginally less than the case of urea and water. The data on the groove width indicate the possibility of different interaction of Major & Minor Grooves. Major & Minor Grooves in a space-filling model of DNA. The major and minor grooves are opposite each other, and each runs continuously along the entire length of the DNA molecule. They arise from the antiparallel arrangement of the two backbone strands DNA major and minor grooves. Daniel Fried. K subscribers. Subscribed. 1K. K views 8 years ago. My teaching project page: Biochemistry Literacy for Kids DNA minor groove is the target: the small molecules' attack. Go to: Abstract. The macromolecule that carries genetic information, DNA, is considered as an exceptional target for diseases depending on cellular division of malignant cells (i.e. cancer), microbes (i.e. bacteria) or parasites (i.e. protozoa) DNA grooves. Two major and one minor groove are shown in this space-filling model of DNA. Full size image. Oligonucleotides may recognize the double helix and form triple helix structures but do not provide sufficient single base (pair) specificity DNA structure and function. DNA is the information molecule. It stores instructions for making other large molecules, called proteins. These instructions are stored inside each of your cells, distributed among 46 long structures called chromosomes. These chromosomes are made up of thousands of shorter segments of DNA, called genes
Discovery of the structure of DNA (article) | Khan Academy
Major groove. Major groove. in a detailed analysis of dna structure, there are two types of grooves that can be seen; the major groove has the nitrogen and oxygen atoms of the base pairs pointing inward toward the helical axis, while in the minor groove, the nitrogen and oxygen atoms point outwards; important because the major groove is The functional groups of minor groove stabilized DNA helix and A/B equilibrium in a cooperative manner. The equilibrium is shifted from B to A form in absence of functional groups of adenine and The major groove is wider than the minor groove in DNA (Figure \(\PageIndex{2d}\)), and many sequence specific proteins interact in the major groove. The N7 and C6 groups of purines and the C4 and C5 groups of pyrimidines face into the major groove, thus they can make specific contacts with amino acids in DNA-binding proteins Major and minor groove triplexes. The minor groove triplex is a ubiquitous RNA structural [HOST]e interactions with the minor groove are often mediated by the 2'-OH of the ribose sugar, this RNA motif looks very different from its DNA equivalent. The most common example of a minor groove triple is the A-minor motif, or the insertion of adenosine